Write down the OECD tests used for testing of new chemicals?
- Acute Oral test
- Reproductive test + Developmental studies
- Inhalation test
- Skin Sensitivity test
- Carcinogenicity
- Mutagenicity
- Immunotoxicity
- SubChronic and Chronic tests
Describe Chromatographic Determination of Alfatoxins in the Biological Material
- Chloroform is added to 10g of Fodder
- Alfatoxins are Extracted by Shaking = Cold Extraction
- Filter and Thicken = 1-2ml
- Place a drop of Sample and Standard on Chromatographic plate
- Develope in Chloroform and Aceton compound
- Stop developin when solvent has reached the front
- Dry it!
- Observe under UV light
- Results:
-B1 and B2= Blue Fluorescence
-G1 and G2= Green Fluorescence
Write down the Clinical Significance of Enzyme Determination
- Diagnosis of Subclinical disease
- Diagnosis of disease when manifesting phase has only the common symptoms
- Determination of the degree of organ damage
- Determination of prognosis and efficiency of therapy according to the time dependent dynamics of enzyme levels
Describe screening method for detection of Aspergillus Flavus in Cereals
Determination of Mold and Alfatoxins
* Sample: Whole grain
* Grain is placed in 15x15 frame
* Placed under UV light in dark room
* The Aspergillus will Fluorescence green
* Count the grain with green on them
Evaluation
* 0-1 grain = Negative results
* 2-3 grains = Suitable for animal feeding only
* 4+ grains = Unconditionally the grains must further analyzed for Quantitative determination of Alfatoxins
Solution to fix contaminated feed = Mix with uncontaminated feed, will dilute the %
