purpose of enzymes
to speed up chemical reactions in the body
how enzymes work
weakens bonds and lowers activation energy (the energy needed to start a chemical reaction)
uses of enzymes in the body
lipase is produced by the pancreas and used for fat digestion
uses of enzymes in the industry
lactase is used to make lactose free diary products
induced fit model
active site changes slightly to fit the substrate as it binds
5 factors affecting enzyme activity
temperature and enzymes
optimum temperature is 37-45 deg celcius
below: colder molecules move more slowly, resulting in less collisions
optimum: higher rates of enzyme-substrate complex
above: active site changes as heat changes the 3d structure
enzyme substrate complex
the state where an enzyme is attaching to a substrate
ph and enzymes
enzyme denatures when its too high or too low
concentration of substrate/enzyme affection on function
as the concentration is higher, the rates of reaction increases
cofactors and coenzymes effect on enzyme function
inorganic substances and vitamins are needed to bind to an enzyme to make it active
enzyme inhibitors effect on enzyme function
competitive: chemicals that resemble the substrate enough so they can attach to the active site -> blocks actual substrate from binding
noncompetitive: doesn’t enter the active site, binds to another part so that the active site changes shape
allosteric sites
binds to another molecule to slow/stop enzyme activity
positive feedback
product of a reaction naturally inc enzyme activity
negative feedback
final product of a pathway inhibits an enzyme earlier in the pathway
diabetes
eihter the body cant produce insulin or it cant use insulin, cant regulate sugar in bloodstream
glycemic load
measures the food impact on blood sugar from the total carbs plus glycemic index
passive transport
no energy required
active transport
energy required