3.8.4.2

Question 1

What are DNA probes?

Answer 1

Single stranded pieces of DNA that are complimentary and bind to certain base sequences.

Question 2

What are all probes labelled with?

Answer 2

Radioactive markers

Question 3

Uses of DNA probes

Answer 3

Locate a specific allele of a gene

To see if a person carries a mutated allele

Help determine how people will react to drugs

Identify health risks

Question 4

Method of using DNA probes

Answer 4

DNA sample digested into fragments using restriction enzymes and seperated with gel electrophoresis

DNA fragments transferred to nylon membrane and incubated with DNA probe with marker.

Array is washed.

Bands with the DNA proble will be marked and present.

Question 5

How do DNA probes help genetic counselling?

Answer 5

Informed decisions about genetics and so healthcare can be advised on.

Question 6

How do DNA probes help personalised medicine?

Answer 6

Different dosages or compounds used depending on the genetic constitution