The process that uses centrifugal force for the separation of two liquids in a mixture.
Centrifugation
[3] Variables
- Mass
- Speed
- Radius
Process in which centrifugal force is used to separate solid matter from a liquid suspension.
Centrifugation
Indicates speed
Tachometer
Revolutions per minute (rpm)
Speed
RCF =
1.118.10 r (rpm)
Measured from the center of the centrifuge axis to the bottom of the test tube shield or bucket.
R (radius)
AKA swinging bucket
Horizontal Head Centrifuge
The cups are held in a RIGID POSITION at a fixed angle.
Fixed Angle Head Centrifuge
Less sediment disturbance
Fixed Angle Head Centrifuge
Uses a very HIGH - TORQUE and LOW - INERTIA motor to spread monolayers of cells rapidly across a special slide for critical morphologic studies.
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Cytocentrifuge
Refrigerated centrifuges:
−15°C to −25°C
This permits centrifugation at higher speeds as specimens are protected from the heat generated by the rotors of the centrifuge.
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Refrigerated Centrifuges
Are high-speed centrifuges generally used for research projects.
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Ultracentrifuges
Operates at 90,000 to 100,000 rpm.
[centrifuge]
Ultracentrifuges
Generates a maximum RCF of 178,000 g.
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Ultracentrifuges
Used to set the desired speed.
Rheostat
Control the flow of electric current by manually increasing or decreasing the resistance.
Rheostat
Revolutions per minute. Speed of centrifugation. Determined by tachometer
[centrifuge]
rpm
Distance in cm from center of rotation to bottom of tube when rotating.
[centrifuge]
Radius (r)
Tubes are at fixed angle (25° - 40°) when rotating. CAPABLE FO HIGHER SPEED.
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Angle head centrifuge
Produces a slanted sediment surface that isn’t tightly packed. Decantation is NOT recommended.
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Angle head centrifuge
High speed. Capable of 100, 000 rpm. Refrigerated to reduce heat.
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Ultracentrifuge
FORCE ACTING on sample being centrifuged. Gravities (g).
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Relative Centrifugal Force (RCF)
