Micropipetting

Question 1

The MOST ROUTINELY USED pipetted in the molecular laboratory.

Answer 1

Automatic pipette

Question 2

They used to measure and transfer volumes of liquid accurately (microscale).

Answer 2

Automatic pipette

Question 3

When aspirating and dispensing fluids, maintain angles closest to vertical. Angles GREATER THAN 20° of vertical will affect results.

Answer 3

Angles

Question 4

When aspirating fluids, only immerse, the tip as LITTEL AS POSSIBLE, while making sure to completely aspirate the full volume.

Answer 4

Immersion

Question 5

Remove adhering liquid by brushing the tip against the rim of the primary vessel.

Answer 5

Wipe

Question 6

For AQUEOUS SOLUTIONS, radioactive compounds, acid/alkalis, and nucleotide solutions; which may require MIXING following dispensing. Forms FOAMS and BUBBLES.

[pipetting technique]

Answer 6

Forward pipetting

Question 7

For precise pipetting of SMALL VOLUMES, pipetting of highly VISCOUS or VOLATILE compounds, and solutions that TEND TO FOAM or splash.

[pipetting technique]

Answer 7

Reverse pipetting

Question 8

For precise repetitive pipetting of the SAME VOLUME in which carry over is not concern.

[pipetting technique]

Answer 8

Repetitive pipetting

Question 9

For dispensing full amounts of HETEROGENOUS SAMPLES such as whole blood and serum.

[pipetting technique]

Answer 9

Pipetting of Heterogenous Samples

Question 10

Neutralizes capillary effect and equilibrates the temperature between the air cushion, pipet tip, and the liquid itself.

[pipetting technique]

Answer 10

Pre-wetting