Pipetting

Question 1

[5] Types of glassware

Answer 1

1. Borosilicate glass
2. Boron-free glassware
3. Corex (corning)
4. Vycor (corning)
5. Flint glass

Question 2

Most commonly used.

[glassware]

Answer 2

Borosilicate glass

Question 3

Used for HEATING and STERILIZATION.

[glassware]

Answer 3

Borosilicate glass

Question 4

Characterized by a high degree of thermal resistance and has LOW ALKALI content.

[glassware]

Answer 4

Borosilicate glass

Question 5

Its thermal resistance is LESS as compared to borosilicate glass. HIGH resistance to alkali.

[glassware]

Answer 5

Boron-free glassware

Question 6

SPECIAL ALUMINA - silicate glass

[glassware]

Answer 6

Corex

Question 7

Utilized for high thermal, drastic heat shock and extreme chemical treatment with acids and dilute alkali.

[glassware]

Answer 7

Pyrex

Question 8

Made up soda-lime glass and a mixture of CALCIUM, SILICON, and SODIUM OXIDES.

[glassware]

Answer 8

Flint glass

Question 9

POOR RESISTANCE to high temperature.

[glassware]

Answer 9

Flint glass

Question 10

[2] Calibration marks/design

Answer 10

1. To deliver (TD)
2. To contain (TC)

Question 11

[2] Drainage characteristics.

Answer 11

1. Blowout
2. Self-draining

Question 12

[2] Types of pipette

Answer 12

1. Transfer pipette
2. Graduated or measuring pipette

Question 13

Delivers the exact amount it holds onto container.

[calibration marks]

Answer 13

To deliver (TD)

Question 14

Holds the particular volumes but does not dispense the exact volume.

[calibration marks]

Answer 14

To contain (TC)

Question 15

WITH continuous etched ring on top of the pipet.

[drainage characteristics]

Answer 15

Blow out

Question 16

Exact volume is obtained when the last drop is blown out.

[drainage characteristics]

Answer 16

Blow out

Question 17

WITHOUT ETCHED ring on top of the pipet.

[drainage characteristics]

Answer 17

Self draining

Question 18

Liquid is allowed to drain by gravity.

[drainage characteristics]

Answer 18

Self draining

Question 19

[4] Transfer pipette

Answer 19

1. Volumetric pipette
2. Ostwald folin
3. Pasteur pipette
4. Automatic micro/macro pipette

Question 20

[5] Graduated or Measuring pipette

Answer 20

1. Serological pipette
2. Mohr pipette
3. Bacteriologic pipette
4. Ball, Kohmer and Kahn
5. Micropipettes

Question 21

Self draining & NONVISCOUS fluid.

[transfer pipette]

Answer 21

Volumetric pipette

Question 22

WITH ETCHED ring & VISCOUS fluid.

[transfer pipette]

Answer 22

Ostwald folin

Question 23

Commonly made from plastic and transfer fluid without consideration of specific volume.

[transfer pipette]

Answer 23

Pasteur pipette

Question 24

[4] Micropipettes

Answer 24

1. Sahli - Helige pipette
2. Lang - levy pipette
3. RBC and WBC pipette
4. Kirk and Overflow pipette

Question 25

[3] Classification according to mechanism

Answer 25

1. Air - displacement 2. Positive displacement 3. Dispenser/dilutor

Question 26

It RELIES ON PISTON for suction creation to draw sample into a disposable tip. [classification according to mechanism]

Answer 26

Air - displacement

Question 27

Piston does not come in contact with the liquid. [classification according to mechanism]

Answer 27

Air - displacement

Question 28

It operates by MOVING THE PISTON in the pipet tip or barrel, like hypodermic syrine. [classification according to mechanism]

Answer 28

Positive displacement

Question 29

It obtains liquid from a common reservoir and dispense it repeatedly. [classification according to mechanism]

Answer 29

Dispenser/Dilutor

Question 30

It combines SAMPLING and DISPENSING functions. [classification according to mechanism]

Answer 30

Dispense/Dilutor