[6] Enzymatic Cholesterol Reagent
- 4 - Aminophenazone
- Phenol
- Peroxidase
- Cholesterol Esterase
- Cholesterol Oxidase
- Buffers and Stabilizers
Reagent and Standard stable stored at
2 - 8℃
Blood should be collected following a
12 - hour fast
Both total cholesterol and HDL cholesterol are reportedly stable at
4 days at 2 - 8℃
Piper into cuvets the following volumes (mL) and mix well.
[steps]
Step 1
Incubate all cuvets at 37℃ for 5 minutes or at room temperature for 10 minutes.
[steps]
Step 2
Read S and U vs. RB at 500 nm within 60 minutes.
[steps]
Step 3
[2] Anticoagulants that will result false low values.
- Fluoride
- Oxalate
Incubate all cuvets at ___ for 5 minutes at room temperature for 10 minutes.
37 ℃
Wavelength
500 nm
Reaction type
Endpoint
Reaction direction
Increasing
Reaction temperature
37 ℃
Sample/Reagent Ratio
1:100
Equilibration time
3 seconds
Read time
4 seconds
Lag time
300 seconds
Blank Absorption Limit
0.30 A
Standard
200 mg/dL
High Absorbance
2.000 A
Low normal
120 mg/dL
High normal
300 mg/dL
Linearity
750 mg/dL
Cord
[total cholesterol]
45 - 100
