CH 3 lecture

Question 1

a culture which contains a single
species of microorganism

Answer 1

PURE (axenic) CULTURE

Question 2

increasing the population of
microorganisms by providing
their nutritional and physical
requirements

Answer 2

CULTIVATION

Question 3

extracellular substances which provide the cell with materials for building protoplasm and for energy generation

Answer 3

NUTRIENTS

Question 4

CULTURE MEDIUM uses (3)
- g___ and m___
- favor the p___ of particular compounds
- study m___ action

Answer 4

GROWTH AND MAINTENANCE
PRODUCTION
MICROBIAL

Question 5

% of Solidifying Agent of:
1. Liquid
2. Semi-Solid
3. Solid

Answer 5

1.) 0
2.) 0.1 - 0.5
3.) 1.5 - 2.0

Question 6

2 most used TYPES of SOLIDIFYING AGENT

Answer 6

Agar and Gelatin

Question 7

TYPE of culture media acc to CHEMICAL COMPOSITION:

1. all components are
   chemically defined
2. NOT all components are
   chemically defined

Answer 7

1. Synthetic
2. Complex

Question 8

types of CULTURE MEDIA according to PURPOSE:

1. distinguishes one type of bacteria from another
2. used to determine qualitative/ quantitative production of such a
   compound by an organism
3. used to increase the number of
   microorganisms with unusual
   physiological characteristics
4. can support most or almost all types of species
5. with special reagents like pH indicators or dyes
6. allows the growth of a specific
   type of microorganism only

Answer 8

1. Differential
2. Assay
3. Enrichment
4. General Purpose
5. Differential
6. Selective

Question 9

EXAMPLES of CULTURE MEDIA according to PURPOSE:
1. Blood Agar
2. Nutrient Agar
3. Eosin Methylene Blue Agar
4. Potato Dextrose Agar
5. Triple Sugar Iron Medium
6. Bacillus Cereus Agar
7. Fermentation Medium

Answer 9

1. Enrichment
2. General Purpose
3. Differential
4. General Purpose
5. Assay
6. Selective
7. Assay

Question 10

TYPES of ISOLATION TECHNIQUES

1. a macroscopically visible
   growth or cluster of
   microorganisms on a solid
   medium
2. used if the desired microorganism is present at a higher level than
   any other microorganism
3. isolation of specific types of
   microorganisms by a
   combination of nutrient and
   physical conditions
4. for samples with low population: uses a sterile membrane filter having a pore size that retains microorganism
5. uses a micropipette or a microprobe to physically pick a single cell and transfer it on an agar medium
6. used for the isolation of unusual
   physiological types of
   microorganisms which are
   present in small numbers and
   which grow slowly

Answer 10

1. Plating
2. Serial Dilution
3. Enrichment Culture
4. Membrane Filter Technique
5. Single-cell Isolation Technique
6. Enrichment Culture

Question 11

3 Types of PLATING

Answer 11

1. Streak Plating
2. Spread-Plate Method
3. Pour-Plate Method