Why can the free energy change (ΔG) differ from the standard state value (ΔG°)?
Because actual concentrations of reactants and products often differ from 1 M
Why is the standard state of 1 M H+ not useful in biochemistry?
Because it implies pH 0, which is not typical in biological systems.
What modified standard state is used in biochemistry?
1 M for all solutes except protons, with pH fixed at 7
How is this biochemical standard state denoted?
With a prime symbol: ΔG′, ΔH′, ΔS′, etc