Chapter 4 section 6

Question 1

What property differences are used to separate amino acids?

Answer 1

Differences in ionization behavior, solubility, and partitioning between phases.

Question 2

What is chromatography?

Answer 2

A separation method based on repeated partitioning of molecules between two phases.

Question 3

Who developed chromatography and when?

Answer 3

Mikhail Tsvet in 1903, for separating plant pigments.

Question 4

Name three chromatography methods used for amino acids.

Answer 4

Ion-exchange chromatography, gas chromatography (GC), high-performance liquid chromatography (HPLC).

Question 5

Why is HPLC commonly used for amino acids?

Answer 5

High resolution, sensitivity, and speed, despite relatively low capacity.

Question 6

What is precolumn derivatization in HPLC?

Answer 6

Amino acids are converted to derivatives (e.g., PTH) for better detection and separation.