Coagulation methods Flashcards by Mr. Z

Q

What anticoagulant is used for coagulation testing?

A

3.2% sodium citrate (binds Ca²⁺)

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Q

What is the correct blood:citrate ratio?

A

9:1

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Q

What happens if hematocrit >55%?

A

Decrease citrate volume

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Q

What plasma is used for clot-based tests?

A

Platelet-poor plasma (PPP)

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Q

What platelet count defines PPP?

A

<10,000/µL

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Q

What plasma is used for platelet aggregation?

A

Platelet-rich plasma (PRP)

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Q

What does platelet aggregation measure?

A

Platelet adhesion, aggregation, secretion

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Q

Principle of light transmission aggregometry?

A

↑ aggregation → ↑ light transmission

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Q

Principle of impedance aggregometry?

A

↑ aggregation → ↑ electrical impedance

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Q

What are common platelet agonists? (4)

A

ADP, collagen, epinephrine, ristocetin

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Q

What is the primary wave?

A

Initial platelet aggregation

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Q

What is the secondary wave

A

Secretion-dependent aggregation

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Q

What does APTT measure?

A

Intrinsic + common pathways

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Q

What does PT measure?

A

Extrinsic + common pathways

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Q

What factors are NOT measured by APTT?

A

VII, XIII

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Q

What therapy does APTT monitor?

Study These Flashcards

A

Heparin

Q

What therapy does PT monitor?

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A

Warfarin

Q

Mixing study corrects → what does that mean?

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A

Factor deficiency

Q

Mixing study does NOT correct → what does that mean?

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A

Inhibitor present

Q

What does thrombin time (TT) measure?

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A

Fibrinogen → fibrin conversion

Q

What interferes with TT?

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A

Heparin

Q

What is reptilase time used for?

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A

Detect fibrinogen issues (not affected by heparin)

Q

Anti-FXa assay measures what?

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A

Heparin activity via factor Xa inhibition

Q

Fibrinogen is produced where?

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A

Liver

Low fibrinogen causes what lab effect?

Prolonged clotting times

Fibrinogen is what type of protein?

Acute phase reactant

vWF antigen measures what?

Quantity

vWF activity measures what?

Function (platelet binding)

Functional assays measure what?

activity

Immunologic assays measure what?

Quantity only

LA prolongs which tests?

Phospholipid-dependent tests (APTT)

Does LA correct with mixing?

no

Optical detection measures what?

Change in light transmission

Mechanical detection measures what?

Physical clot formation

Chromogenic assays measure what?

Color change from enzyme reaction