ISBB - ANTIBODY STRUCTURR AND FUNCTION

Question 1

Determine the isotype/class

Answer 1

2 heavy chains

Question 2

Heavy chains are

Answer 2

Gamma
Alpha
Mu
Delta
Epsilon

Question 3

Light chains are

Answer 3

Kappa and Lambda

Question 4

Light chains in urine

Answer 4

Bence jones protein

Question 5

Consists of 1 L chain and 1/2 Heavy chain held together by disulfide bonds

Answer 5

Fab fragment

Question 6

Consist of heavy chains halves held together by disulfide bonds

Answer 6

Fc fragment

Question 7

Carboxy-terminak ends of H and L chains where amino acid sequence is the same for all chains of that type

Answer 7

Constant region

Question 8

Binds complement specifically C1q and initiates complement activation

Answer 8

CH2

Question 9

Responsibke for cytotropic reaxtions involving macrophages, NK cells and mast cells

Answer 9

CH3

Question 10

Amino-termunal ends of H and L where amino acid sequence varies

Answer 10

Variable region

Question 11

Flexible portion between CH1 and CH2

Answer 11

Hinge region

Question 12

Flexibility of hinge region is attributed to

Answer 12

Proline

Question 13

Yields 2 Fab fragments and Fc; can bind but cannot agglutinate or precipitate

Answer 13

Papain

Question 14

Yields a Fab and Fc ; can bind, agglutinate and precipitate

Answer 14

Pepsin

Question 15

Determined by tge nature of the constant heavy chain

Answer 15

Isotype

Question 16

Genetic variatioj in the constant region in some indivifuals but not others

Answer 16

Allotype

Question 17

Unique amino acid sequences in the variable region that give the antibofy its specificity

Answer 17

Idiotype

Question 18

Certain cells express receptors before antigeb contact which break off on activationand enter the circulation as antibodies

Answer 18

Side chain theory

Question 19

Lymphocytes are pre endowned to respond to an antigen, with IgM and IgD acting as receptirs that interact with specific antigen to trigger proliferation of a clone of identical cells

Answer 19

Clonal selection theory

Question 20

Spleen B cells from an immunized mouse are fused with myeloma cells producing monoclonal antibodies

Answer 20

Hybridoma technology

Question 21

Gene rearrangements occur during the maturatiin of B cells thereby generating diversity of B cell receptors and antibodies

Answer 21

Somatic recombination

Question 22

All IgG subclasses activates complement except

Answer 22

IgG4

Question 22

The only immunoglobulin that has a 4 CH domains (All have 3, IgM don’t have)

Answer 22

IgE

Question 22

Immunoglobulin that has a longest half life (23 days)

Answer 22

IgG

Question 22

Major Immunoglobulin in Secondary/Anamnestic

Answer 22

IgG

Question 23

The only immunoglobulin that has a svedberg value of 19s (all are 7s,8s)

Answer 23

IgM

Question 24

All subclasses of IgG can cross placenta except

Answer 24

IgG2

Question 25

Immunoglobulin that activates complement

Answer 25

IgG and Igm

Question 25

Only immunoglocbulin produced in newborns

Answer 25

IgM

Question 26

More efficient at agglutination than IgG

Answer 26

IgM

Question 27

Monomer units of IgM are held togther by?

Answer 27

J chains

Question 28

Secretory IgA has a

Answer 28

J chain and Secretor piece

Question 28

IgA is ____ in secretions

Answer 28

Dimer

Question 29

Preventd adherence of bacteria to mucosa and neutralizes toxins

Answer 29

IgA

Question 29

IgA is ____ in serum

Answer 29

Monomer

Question 30

Reaginic antibody associated in type l HS reaction

Answer 30

IgE

Question 30

IgE binds to Fc receptor on

Answer 30

Basophiks and Mast cells

Question 31

Naive B cell receptor

Answer 31

IgD

Question 32

Only IgG that has NO resction with protein A

Answer 32

IgG3

Question 32

Most abundant IgG

Answer 32

IgG1

Question 32

Susceptible to enzyme degradatioj and is heat and acid labile

Answer 32

IgD

Question 33

IgG subclasses are distinguised/differ mainly in

Answer 33

Disulfide bond arrangement Size of hinge region Decrasing average serum concentrations