VIRAL REPLICATION

Question 1

RECOGNITION AND ATTACHMENT

1. Rhabodyviridae
   Rabies Virus
   Viral protein attachment: __________

Answer 1

G protein gp

Question 2

RECOGNITION AND ATTACHMENT
Virus Family: Paramyxoviridae
Virus: Measles
Viral Attachment Protein: _______

Answer 2

HAgp

Question 3

RECOGNITION AND ATTACHMENT
EXAMPLES OF VIRAL RECEPTORS
Virus: Rabies virus
Target Cell: Neuron
Receptor: _____________

Answer 3

Acetylcholine Receptor, NCAM

Question 4

ATTACHMENT AND ADSORPTION
It is a 1.) __________; does not require energy

2 Requirements:
1. Recognition and Attachment to specific host receptor -> determines host range
2. Appropriate 2.)_____&______:
ph 7 and counter ion (magnesium ion from host cell)

Answer 4

1. reversible step
2. appropriate pH and ionic concentration

Question 5

STEPS IN VIRAL REPLICATION
ATTACHMENT AND ADSORPTION

Examples of viruses and their cell surface receptors:
1. Influenza virus - ________
2. Hepatitis C - __________
3. Hepa B - _____
4. Rabies - _______
5. SARS-CoV2 - _________

Answer 5

1. Sialic Acid
2. LDL Receptor
3. acetylcholine receptor
4. IgA receptor
5. ACE-2 receptor

Question 6

PENETRATION
MODE OF ENTRY
ENTRY OF ENVELOPED VIRUSES

1.)____________
-> Viral attachment to host membrane
->Viral ligand and not the cellular receptor mediates fusion
-> pH independent process

EXAMPLE: Measles and mumps virus

Answer 6

ligand mediated fusion

Question 7

PENETRATION
MODE OF ENTRY
ENTRY OF ENVELOPED VIRUSES

2.)____________
1. Virus binds to cell surface
2. receptor formation of clathrin-coated pit
3. clathrin-coated pit encases virus
clathrin-coated vesicle formed
4. clathrin coat shed off
5. virus-containing vesicle fuse with lysosome
6. capsid dissociates
7. release of viral genome

Answer 7

ENTRY OF NON-ENVELOPED VIRUSES uses clathrin

Question 8

RNA VIRUSES
Replicate in the cytoplasm EXCEPT influenza and retrovirus

1.) ss RNA of Positive Polarity
1. RNA genome used directly as _________
2. with the same base sequence as the mRNA

example: Picorna, Flavi, Retro, Corona

Answer 8

mRNA

Question 9

RNA VIRUSES
Replicate in the cytoplasm EXCEPT influenza and retrovirus

2. Ss RNA of Negative Polarity
   Virus encodes its own RNA-dependent RNA polymerase converted to ________

Answer 9

Positive Polarity

Question 10

RNA VIRUSES
Replicate in the cytoplasm EXCEPT influenza and retrovirus

3.__________
Host does not produce RNA-dependent polymerase which becomes viral RNA

Virus carries own RNA-dependent RNA polymerase

Answer 10

Double Stranded RNA

Question 11

RNA VIRUSES
Replicate in the cytoplasm EXCEPT influenza and retrovirus

1. Carries RNA-dependent DNA polymerase (reverse transcriptase)
2. ds DNA
3. transcribed to mRNA by host cell polymerase

Answer 11

ssRNA with positive Polarity and DNA intermediate