- Molecular techniques are being used to detect abnormalities of
A. erythrocytes
B. leukocytes
C. some coagulation factors
D. All of the above
D. All of the above
- The first inherited hematologic disorder to be diagnosed using molecular biologic assay was
A. hemophilia A
B. factor V Leiden
c. sickle cell anemia
D. CML
c. sickle cell anemia
- PCR testing is useful in
A. forensic testing
B. genetic testing
C. disease diagnosis
D. All of the above
D. All of the above
- The traditional PCR technique
A. extends the length of the genomic DNA
B. alters the original DNA nucleotide sequence
C. amplifies low levels of specific DNA sequences
D. amplifies the target region of RNA
C. amplifies low levels of specific DNA sequences
- PCR protocol
A. doubles the specific amount of DNA with each cycle
B. typically has three temperature steps
C. repeats the number of cycles about 30
D. all of the above
D. all of the above
- Variations of PCR include
A. nested primers
B. real-time PCR
C. Microarray analysis
D. Both A and B
D. Both A and B
- The method that continues to be considered the “gold standard* of DNA sequencing
A. Sanger method
B. Southern blot method
C. Northern blot method
D. Dot blot method
A. Sanger method
- The Southern blot procedure has diagnostic applications for diseases or disorders associated with
A. significant changes in DNA (e.g., deletion)
B. determination of clonality in lymphomas of T- or B-cell origin
C. detection of restriction fragment length polymorphisms
D. all of the above
D. all of the above
- All of the following are true of FISH except
A. The acronym stands for fluorescent in situ hybridization.
B. It is a tissue-based molecular diagnostic assay.
C. It is a prenatal diagnosis of a genetic disorder.
D. It is useful in the diagnosis of various anemias.
D. It is useful in the diagnosis of various anemias.
*10. Microarrays are
A. DNA probes bonded on glass chips
B. tissue-based probes
C. used to identify single-base mutations
D. used to determine clonality in lymphomas
A. DNA probes bonded on glass chips
*11. Molecular techniques provide a diagnostic tool to
A. detect MRD in hematological malignancies
B. monitor patients following bone marrow transplantation
C. detect an early relapse in a patient treated for a hematological malignancy
D. all of the above
D. all of the above
*12. Which of the following best describes the molecular alteration of the Philadelphia chromosome to chronic myelogenous leukemia (CML)?
A. The mutation produces an overly active tyrosine kinase that increases apoptosis.
B. The mutation reduces serine enzyme activity in affected cells.
C. The mutation involves translocation of a gene that is critical to myelocytic maturation.
D. Hematopoietic cells gain a proliferation advantage because of tyrosine kinase activity.
C. The mutation involves translocation of a gene that is critical to myelocytic maturation.
*12. Which of the following best describes the molecular alteration of the Philadelphia chromosome to chronic myelogenous leukemia (CML)?
A. The mutation produces an overly active tyrosine kinase that increases apoptosis.
B. The mutation reduces serine enzyme activity in affected cells.
C. The mutation involves translocation of a gene that is critical to myelocytic maturation.
D. Hematopoietic cells gain a proliferation advantage because of tyrosine kinase activity.
C. The mutation involves translocation of a gene that is critical to myelocytic maturation.
