ALP

Question 1

EC of ALP

Answer 1

E.C. 3.1.3.1

Question 2

a nonspecific enzyme that functions to liberate inorganic phosphate from an organic phosphate ester with the concomitant production of an alcohol

Answer 2

ALP

Question 3

Liver ALP is located on

Answer 3

sinusoidal and bile canalicular membranes.

Question 4

Bone ALP activity confined to

Answer 4

osteoblasts.

Question 5

Major isoenzymes

Answer 5

Liver
Bone
Placental
Intestinal

Question 6

ALP is marker for

Answer 6

obstructive jaundice, hepatic and bone disorders (Paget’s disease)

Question 7

optimum pH for alkaline phosphatase activity is at pH

Answer 7

8.6

Question 8

it is the major liver fraction

Answer 8

ALP

Question 9

Elevated ALP is significant in diagnosing

Answer 9

Hepatobiliary and Bond Disorders

Question 10

Intestinal ALP isoenzyme presence in plasma depends on blood type and secretor status, with blood types ____ and ____ more likely to have this function

Answer 10

Types B and O

Question 11

is a valuable tumor marker for most germ cell tumors in both serum and cerebrospinal fluid (CSF)

Answer 11

Placental alkaline phosphatase (PLAP)

Question 12

GGT is an alcohol sensitive enzyme which can distinguish the organ source of ALP

Liver =
Bone =

Answer 12

Liver = ⬆️ ALP and GGT
Bone = ⬆️ ALP but normal GGT

Question 13

Component of ALP and its enzyme activator

Answer 13

Zinc

Activator: Magnesium

Question 14

Abnormal ALP isoenzymes associated with neoplasms

Answer 14

Regan and Nagao

Question 15

The Regan isoenzyme is ectopically produced by malignant tissue and is useful for monitoring therapy success; heat stable and inhibited by _____

Answer 15

Phenylalanine

Question 16

Nagao isoenzyme, a variant, is also inhibited by _____ and found in metastatic cancers such as pancreatic adenocarcinoma

Answer 16

L-Leucine

Question 17

ALP is inhibited by phosphorus; the addition of 2-amino-2-methyl-1-propanol (AMP) buffermbinds phosphorus under

Answer 17

Bowers-McComb method

Question 18

may cause elevations because ALP is approx 6x more concentrated in erythrocytes than in serum

Answer 18

Hemolysis

Question 19

A continuous monitoring technique that uses para-nitrophenyl phosphate as a substrate for the determination of alkaline phosphatase activity and allows calculation of ALP activity based on the molar absorptivity of para-nitrophenol

Answer 19

Bessey-Lowry-Brock method

Question 20

Most useful single technique for distinguishing between ALP isoenzymes

Answer 20

Electrophoresis

Question 21

Most anodal to least anodal

Answer 21

Land Bank of the Philippine Island

Liver > Bone > Placental > Intestine

Question 22

Use of ____ and ____ improves separation of bone and liver ALPs

Answer 22

Neuraminidase and wheat germ lectin

Question 23

Heat denaturation is determined by heating serum at

Answer 23

56°C for 10-15 mins

Question 24

Most heat stable

Answer 24

Placental

Question 25

Most heat labile

Answer 25

Bone

Question 26

Most heat stable among all type

Answer 26

Regan

Question 27

Inhibits placental, intestinal, regan, and nagao

Answer 27

L-phenylalanine

Question 28

Inhibits liver and bone isoenzymes

Answer 28

Levamisole

Question 29

Bone isoenzyme and liver

Answer 29

L-homoarginine

Question 30

Inhibits bone isoenzyme

Answer 30

2M urea

Question 31

Inhibits nagao isoenzyme

Answer 31

L-leucine

Question 32

Denatures liver ALP rapidly than bone

Answer 32

20% ethanol

Question 33

Substrate: Beta-glyceroPO4 End product: Inorganic PO4 + Glycerol

Answer 33

1. Bodansky 2. Shinowara 3. Jones 4. Reinhart

Question 34

Substrate: Phenylphosphate End products: Phenol

Answer 34

King and Armstrong

Question 35

Substrate: p-nitro phenyl PO4 (PNPP) End product: p-nitrophenolor yellow nitrophenoxide ion

Answer 35

1. Bessy, Lowry & Brock 2. Bowers and McComb

Question 36

Substrate: Phenolphthalein diphosphate End product: Phenolphthalein red

Answer 36

Huggins and Talalay

Question 37

Substrate: Alpha naphthol PO4 End product: Alpha-naphtol

Answer 37

Moss

Question 38

Substrate: Buffered phenolphthalein PO4 End product: Free phenolphthalein

Answer 38

Klein, Babson & Read