Preanalysis

Question 1

Increased levels if prolonged tourniquet application

Answer 1

Protein (Albumin), Enzymes, Lactate, Cholesterol, Potassium, NH3

Question 2

Prolonged application of tourniquet results to:

Answer 2

Hemoconcentration
Anaerobiosis

Question 3

Exercise increases these in tests

Answer 3

lactate, fatty acid, ammonia, ALT, AST, CPK, LD.

Question 4

These are increased in Exercise

Answer 4

growth hormone, prolactin, testosterone, LH

Question 5

Exercise elevates levels of

Answer 5

Abumin in urine (Albuminuria)

Question 6

Fist clenching increases

Answer 6

Potassium
Lactate
Phosphate

Question 7

Fasting for blood glucose

Answer 7

8-10 hours

Question 8

Fasting for lipid panel

Answer 8

10-12 hours

Question 9

Serum bilirubin may increase after ____ of fasting

Answer 9

After 48 hours

Question 10

Plasma TAG increases after ____ of fasting

Answer 10

After 72 hours

Question 11

Basal state collection

Answer 11

Glucose, cholesterol, TAG, and Electrolytes

Question 12

High protein diet increases

Answer 12

Urea

Question 13

Increases turbidity or lactescence

Answer 13

TAG levels exceed 400 mg/dL

Question 14

Preferred position

Answer 14

Upright and supine position (for at least 20 mins)

Question 15

Prolonged bedrest results in _____ due to fluid retention

Answer 15

Decreased plasma albumin

Question 16

Significant elevation of _____ after 30 mins of standing due to the release of this from the muscles

Answer 16

Potassium

Question 17

Alcohol use increases

Answer 17

Gamma Glutamyl Transferase and HDL concentration

Question 18

Drugs that can elevate liver function enzymes

Answer 18

Hepatotoxic drugs

Question 19

Diuretics can decrease

Answer 19

Plasma sodium and potassium levels

Question 20

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS

Cortisol

Answer 20

Peaks 4-6 am
Lowest 8pm-12am
Lower @ 8pm than 8 am
Increased with stress

Question 21

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS

Adrenocorticotropic Hormone

Answer 21

Lower at night, increased with stress

Question 22

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS

Plasma Renin Activity

Answer 22

Lower at night, higher than standing supine

Question 23

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS

Aldosterone

Answer 23

Lower at night

Question 24

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS

Insulin

Answer 24

Lower at night

Question 25

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS Growth hormone

Answer 25

Higher in the afternoon and evening

Question 26

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS Acid Phosphatase

Answer 26

Higher in the afternoon and evening

Question 27

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS Thyroxine

Answer 27

Increases with exercise

Question 28

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS Prolactin

Answer 28

Higher with stress, higher levels at 4 and 8 am and 8 and 10 pm

Question 29

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS Iron

Answer 29

Peaks early to late morning Decreases up to 30% during the day

Question 30

TESTS AFFECTED BY DIURNAL VARIATION, POSTURE AND STRESS Calcium

Answer 30

4% decrease supine

Question 31

Selected arteries for Arterial puncture

Answer 31

Radial Brachial Scalp Umbilical Femoral

Question 32

Must perform before blood collection in arterial puncture

Answer 32

Modified Allen Test

Question 33

Tourniquet is applied to

Answer 33

Obstruct the return of blood to the heart and distended the veins

Question 34

If blood pressure cuff is used

Answer 34

60 mmHg

Question 35

For ethanol testing, ____ should be used

Answer 35

Banzalkonium chloride

Question 36

Traumatic draw will result in an increase in

Answer 36

CK Myoglobin Potassium

Question 37

10% contamination with 5% dextrose will increase ____ in a blood sample by ____

Answer 37

Glucose 500 mg/dL

Question 38

early morning blood collection; 12 hrs after the last ingestion of food

Answer 38

Basal state collection

Question 39

Used for BGA (newborn and infants) for measuring pH and pCO, but not pO2

Answer 39

ARTERIAL CAPILLARY BLOOD

Question 40

Preferred for ARTERIAL CAPILLARY BLOOD because of vascularity

Answer 40

Earlobe

Question 41

ARTERIAL CAPILLARY BLOOD most commonly used

Answer 41

Lateral plantar heel surface

Question 42

Excessive centrifugation (>3000 RCF) may cause

Answer 42

cell lysis and slight elevation of LD and Potassium.

Question 43

LD4 and LD5 isoenzymes (decrease) and alkaline phosphatase (increase) are affected by _____ temperature storage prior to testing

Answer 43

Low

Question 44

Respinning gel tubes can cause increased

Answer 44

potassium.

Question 45

Photosensitive analytes:

Answer 45

• Bilirubin • Beta-carotene Porphyrins * V i t . A a n d B 6 • F o l a t e