Creatinine Kinase

Question 1

Enzyme commission number of CK

Answer 1

E.C. 2.7.3.2

Question 2

CK is also known as

Answer 2

Creatine Phosphokinase or ATP-Creatine-N-Phosphotransferase

Question 3

primarily associated with ATP regeneration in muscle and transport systems

Answer 3

Creatine Kinase

Question 4

Its main function occurs in muscle cells, where it helps store high-energy creatine phosphate

Answer 4

Creatine Kinase

Question 5

Major sources

Answer 5

skeletal muscle, heart muscle, brain tissue

Question 6

CK Isoenzymes: exist a s a dimer with B and M type subunits:

Answer 6

CK-BB (brain type) CK - 1
CK- MB (hybrid type) CK -2
CK-MM (muscle type) CK- 3

Question 7

CK isoenzymes migrates fastest in electrophoresis

Answer 7

CK-BB followed by CK-MB and CK-MM.

Question 8

CK levels are sensitive indicators of

Answer 8

acute myocardial infarction (AMI) and muscular dystrophy

Question 9

Key indicator of myocardial damage

Answer 9

CK-MB

Question 10

Complexes with immunoglobulins, detected in older women, not associated with specific disorders.

Answer 10

Macro-CK

Question 11

Found in muscle, brain, and liver, indicates severe illness if present in serum

Answer 11

Mitochondrial CK (CK-Mi)

Question 12

CK activity in serum is unstable and can be rapidly inactivated due to oxidation of

Answer 12

sulfhydryl groups

Question 13

Oxidation can be prevented or reversed by adding sulfhydryl compounds to the reaction
mixture, such as

Answer 13

N-acetylcysteine, Mercaptoethanol,
Thioglycerol,
Dithiothreitol

Question 14

Reference method; allows visualization of atypical forms.

Answer 14

Electrophoresis

Question 15

Most to least anodal:

Answer 15

CK-BB > CK-MB > CK-MM > Atypical isoenzymes

Question 16

forward/direct method
Enzymes: CK, PK, LDH
Optimum pH at 9.0, measured at 340 nm

Answer 16

Tanzer-Gilbarg Assay

Question 17

reverse/indirect reaction

Enzymes: cK, GGPD, HK

Most commonly used and has 2-6 times faster reaction than forward reaction,
depending on conditions, and has less interference from side reactions

optimum pH at 6.8, measured at 340 nm

Answer 17

Oliver-Rosalki