DNA molecule components
5-carbon sugar, phosphate, bases (a,t,c,g)
Chargaff’S rule
A=t, g= C, composition varies between species
DNA structure
Double stranded anti-parallel helical structure with bases perpendicular to the length of the DNA molecule •stabilized by h-bonds
Pyrimidines
One ring- cytosine and thymine
Purines
2 rings- guanine and adenine
How do polynucleotides form?
0H group on 3rd carbon of one nucleotide reacts with phosphate group on 5’ on another nucleotide (free nucleotides have 3 phosphate groups, but bond between 1 and 2 is broken, releasing energy to allow reaction) to make a phosphodiester bond groups are built 5’ to 3’