Steps from DNA to protein
step 1: transcription, step 2: translation
Transcription
DNA sequence of a gene is copied into a messenger RNA (mRNA) molecule in the nucleus
Translation
the (mRNA) molecule moves to the cytoplasm, where it is read by a ribosome to assemble a specific sequence of amino acids, which then fold into a functional protein
Three classes of RNA
messenger RNA (mRNA) which carries genetic code, transfer RNA (tRNA) which brings amino acids, and ribosomal RNA (rRNA) a key part of the ribosome where proteins are made
Structural differences of DNA and RNA
DNA is double helix while RNA is single stranded
Base paring during DNA replication
A-T, G-C in DNA
Base pairing during RNA
A-U, T-A, G-C going from DNA to RNA
tRNA structure
a distinctive L-shaped 3D structure, folded from a single strand, featuring an anticodon loop to match mRNA codons and a CCA-acceptor stem where the correct amino acid attaches
Three stages of translation
initiation, elongation, and termination
Initiation
where the ribosome, mRNA, and tRNA assemble
Elongation
where amino acids are added one by one to form a polypeptide chain
Termination
when a stop codon is reached, releasing the completed protein and disassembling the complex
Three gene mutation
substitution (swapping one base with another), insertion (adding extra bases), deletion (removing bases)
Mutation rate
the frequency of DNA changes, influenced by spontaneous errors and external agents called mutagens
Mutagens
physical, chemical, or biological agents that cause changes (mutations) in an organism’s DNA
Chromosome
thread-like structures in the nucleus of cells that carry genetic information (DNA) organized into genes
Template
a pattern or guide, often a strand of DNA or RNA, that directs the synthesis of a new, complementary molecule
Replication
the biological process where a cell makes an identical copy of its DNA
Anticodons
three-nucleotide sequence on a transfer RNA (tRNA) molecule that pairs with a complementary three-nucleotide codon on messenger RNA (mRNA) during protein synthesis (translation), ensuring the correct amino acid is added to the growing protein chain
Transcription
when a cell creates mRNA copy from a specific segment of DNA, acting as genetic blueprint to build proteins
Semiconservative
a process where each new DNA molecule contains one original (‘parental’) strand and one newly synthesized strand, effectively ‘conserving’ half of the original DNA in each new copy, ensuring accurate genetic inheritance
Promoter
a specific DNA sequence located near a gene that acts as the starting point for transcription
Exon
a segment of a gene’s DNA that contains the instructions building a protein, which gets kept and joined together in the final mRNA molecule