When and where does DNA replication occur
Occurs in synthesis phase of interphase in the nucleus
which proposal of how dna copied is correct
Semi conservative
-Each new molecule of DNA would contain one strand of yje original complementary strand and one new parent strand
True or false
complementary base pairing allowed it to serve as a template for new complementary strand
TRUE
What was menelson and stahl experiment
they identified between parent and daughter dna strand when its copied
parent nucleotide is heavy nitrogen
new nucleotide is light nitrogen
they centrifuged it to see where the position of the dna is
what is the central dogma
dna does transcription to mRNA and it does translate to become protein
what is a bubble in the dna called
replication bubble
what enzyme relieves tension of the DNA strand
Topoisomerase
how does topoisomerase relieves tension
relieves tension by working ahead of helicase by cutting the sugar phosphate backbone
what enzyme unwinds dna strand
helicase
how does helicase unwind dna
it breaks the hydrogen bonds between complementary base pairs
as it unwinds it forms the replication fork
what prevents dna from joining back together
single stranded binding proteins
what do ssbp do
they are located near the replication fork to prevent dna rejoining
what enzyme is known as bob the builder
DNA polymerase III
how does dna poly 3 add new nucleotides
can only add nucleotides to the 3’ of a growing dna strand
how is a dna strand read
3’ to 5’
how is a dna strand built
5’ to 3’
what is a leading strand
continuous synthesis in the direction of the replication for because dna nucleotides is built from the 5’
what is a lagging strand
Dna strand that is built discontinuously because DNA nucleotides can’t be built from the 3’ so it is built in short segments
what are the short segments of dna in a lagging strand called
okazaki fragmenta
what does Primase do
since dna poly 3 can’t make new dna from scratch RNA primer is used as a starter sequence so that dna poly 3 can add new nucleotides to the 3’
primase is in the form of
RNA (a,u,g,c)
what does dna polymerase 1 do
removes section of rna primer and replace it with dna nucleotides
what does dna polymerase 2 do
it edit,repair and proof read dna
it repair mismatched based
can remove abnormal bases
what joins dna segments together
dna ligase
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Biochemistry42
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Lipids22
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Proteins25
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Enzymes11
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Nucleic acids10
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Carbohydrates23
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Cell membrane25
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Metabolism & energy36
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Aerobic respiration:harvesting chemical energy11
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Glycolysis pathway10
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Genetic Discoveries18
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Structure of DNA20
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DNA replication29
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Transcription30
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Translation19
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Mutation and Gene expression11
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Gene expression22
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Biotechnology9
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Population Ecology62
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Homeostasis intro25
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Neuron and synapse17
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Reflex arc/action potential26
