Gene expression Flashcards

(22 cards)

1
Q

Is every gene expressed

A

No

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2
Q

Why is gene regulation paired with gene expression

A

In order for a gene to be expressed, it has to be regulated

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3
Q

If an hair cell was present in stomach DNA would it be expressed

A

No since it would be wasteful

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4
Q

Why in prokaryotes does transcription and translation happen stimutainously

A

This is due to no nucleus in the prokaryote that in the cytoplasm that these 2 processes happen fast

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5
Q

Why does eukaryotes take longer to do translation and transcription

A

-mRNA traveling through nucleus
-Post transcriptional process

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6
Q

How does gene regulation involve or impact transcription

A

Regulatory proteins (transcription factors) are needed in eukaryotic cells where it can bind to the promoter while other transcription factors can bind there to repress it

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7
Q

Do transcription factors play a role if a gene is expressed

A

Yes

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8
Q

What gene regulator does prokaryote have

A

Operon

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9
Q

What is a operon

A

A regulated cluster of genes along with a promoter and a operator

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10
Q

What is a type of operon

A

Lac operon

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11
Q

How does the represser prevent RNA polymerase from working

A

Binding to a sequence called the operator and since the operator doesn’t let the RNA polymerase work the genes won’t be expressed so it won’t be transcribed into an mRNA

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12
Q

When lactose is around does the bacteria want the enzyme to break down the sugar in dairy

A

Yes it does so the bacteria would turn a gene on to be expressed

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13
Q

How does the lac operon repressor get expressed

A

-A form of lactose binds to the repressor so it get out of the RNA polymerase way so it can transcribe

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14
Q

Can the enzyme for lactose only be expressed when it is around

A

Yes the repressor can’t block when lactose is around

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15
Q

Is an enzyme for lactose made when there is no lactose around

A

No because the repressor binds to the operator

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16
Q

is lac operon a positive or negative control

A

Positive control

17
Q

Does lack of AA tryptophan (TRP) cause an inactive (not blocking) repressor or active (blocking repressor)

A

Inactive repressor as TRP is made continuously

18
Q

What is bound to the operator when there is enough trp

A

a repressor and tryptophan compressor

19
Q

What is an restriction enzyme

A

special types of enzymes from unique bacteria that scietnist use to cut DNA

20
Q

Do restriction enzymes only cut at a specific sequence

21
Q

What is unique about the sequences used to cut the DNA

A

they are palindromic sequences (identical backwards)