Is every gene expressed
No
Why is gene regulation paired with gene expression
In order for a gene to be expressed, it has to be regulated
If an hair cell was present in stomach DNA would it be expressed
No since it would be wasteful
Why in prokaryotes does transcription and translation happen stimutainously
This is due to no nucleus in the prokaryote that in the cytoplasm that these 2 processes happen fast
Why does eukaryotes take longer to do translation and transcription
-mRNA traveling through nucleus
-Post transcriptional process
How does gene regulation involve or impact transcription
Regulatory proteins (transcription factors) are needed in eukaryotic cells where it can bind to the promoter while other transcription factors can bind there to repress it
Do transcription factors play a role if a gene is expressed
Yes
What gene regulator does prokaryote have
Operon
What is a operon
A regulated cluster of genes along with a promoter and a operator
What is a type of operon
Lac operon
How does the represser prevent RNA polymerase from working
Binding to a sequence called the operator and since the operator doesn’t let the RNA polymerase work the genes won’t be expressed so it won’t be transcribed into an mRNA
When lactose is around does the bacteria want the enzyme to break down the sugar in dairy
Yes it does so the bacteria would turn a gene on to be expressed
How does the lac operon repressor get expressed
-A form of lactose binds to the repressor so it get out of the RNA polymerase way so it can transcribe
Can the enzyme for lactose only be expressed when it is around
Yes the repressor can’t block when lactose is around
Is an enzyme for lactose made when there is no lactose around
No because the repressor binds to the operator
is lac operon a positive or negative control
Positive control
Does lack of AA tryptophan (TRP) cause an inactive (not blocking) repressor or active (blocking repressor)
Inactive repressor as TRP is made continuously
What is bound to the operator when there is enough trp
a repressor and tryptophan compressor
What is an restriction enzyme
special types of enzymes from unique bacteria that scietnist use to cut DNA
Do restriction enzymes only cut at a specific sequence
Yes
What is unique about the sequences used to cut the DNA
they are palindromic sequences (identical backwards)