Translation Flashcards

From RNA to protein (19 cards)

1
Q

What are the units that make up ribosomes

A

Large subunit and small subunit

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2
Q

What is the function of ribosomes

A

protein production

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3
Q

What are the two types of ribosomes

A

Free ribosome: in the cytosol (synthesize proteins that function in cytosol)
Bound ribosomes: attached to endoplasmic reticulum (synthesise proteins for export or membranes)

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4
Q

What are codons in translation

A

Block of 3 nucleotides decoded into sequence of amino acids

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5
Q

Are transcription and translation simultaneous in prokaryotes

A

Yes because DNA is in the cytoplasm, there’s no mana editing, and ribosomes reads mRNA as its being transcribed

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6
Q

What is the difference in translation in prokaryotes and eukaryotes

A

Time and physical separation between processes

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7
Q

How long does it take eukaryotes to go from DNA to protein

A

1 hour

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8
Q

What shape is a tRNA structure

A

cover leaf structure

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9
Q

What is special about the clover leaf structure

A

anticodon on the “clover leaf” end (the curve)
amino acid attached on the 3’

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10
Q

What is the only enzyme (to know) in translation

A

Amino acyl tRNA synthetase

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11
Q

Why is it good the tRNA amino acid bond is unstable

A

Makes it easy for the tRNA to later give up the amino acid to a growing polypeptide chain in a ribosome

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12
Q

How does ribosomes facilitate during translation

A

facilitates coupling of tRNA anticodon to mRNA codon

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13
Q

What are the different sites on the large subunit

A

A site (arrival site)
P site (processing site)
E site (exit site)

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14
Q

What is the point of the A site

A

Holds tRNA carrying next amino acid to be added to the chain

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15
Q

What is the point of the P site

A

Holds tRNA carrying growing polypeptide chain

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16
Q

What is the point of the E site

A

Empty tRNA
leave ribosomes from exit site

17
Q

What happens during the initiation process

A

brings together mRNA, ribosome subunits, initiator tRNA

18
Q

what happens during elongation

A

adding amino acids based on codon sequence