What are the units that make up ribosomes
Large subunit and small subunit
What is the function of ribosomes
protein production
What are the two types of ribosomes
Free ribosome: in the cytosol (synthesize proteins that function in cytosol)
Bound ribosomes: attached to endoplasmic reticulum (synthesise proteins for export or membranes)
What are codons in translation
Block of 3 nucleotides decoded into sequence of amino acids
Are transcription and translation simultaneous in prokaryotes
Yes because DNA is in the cytoplasm, there’s no mana editing, and ribosomes reads mRNA as its being transcribed
What is the difference in translation in prokaryotes and eukaryotes
Time and physical separation between processes
How long does it take eukaryotes to go from DNA to protein
1 hour
What shape is a tRNA structure
cover leaf structure
What is special about the clover leaf structure
anticodon on the “clover leaf” end (the curve)
amino acid attached on the 3’
What is the only enzyme (to know) in translation
Amino acyl tRNA synthetase
Why is it good the tRNA amino acid bond is unstable
Makes it easy for the tRNA to later give up the amino acid to a growing polypeptide chain in a ribosome
How does ribosomes facilitate during translation
facilitates coupling of tRNA anticodon to mRNA codon
What are the different sites on the large subunit
A site (arrival site)
P site (processing site)
E site (exit site)
What is the point of the A site
Holds tRNA carrying next amino acid to be added to the chain
What is the point of the P site
Holds tRNA carrying growing polypeptide chain
What is the point of the E site
Empty tRNA
leave ribosomes from exit site
What happens during the initiation process
brings together mRNA, ribosome subunits, initiator tRNA
what happens during elongation
adding amino acids based on codon sequence