Enzyme Lab

Question 1

What enzyme breaks down lactose into galactose and lactose

Answer 1

Lactase

Question 2

Is there more activation energy involved in a catalyzed or uncatalyzed reaction?

Answer 2

Catalyzed, they speed up the reaction by lowering the activation energy.

Question 3

Optimal

Answer 3

Best, most favorable, most likely to bring success

Question 4

Where do enzymes function the best?

Answer 4

where they are typically used and found

Question 5

Denaturation

Answer 5

a process in which a protein loses its native structures due to the disruption of weak chemical bonds and interactions

Question 6

What are extreme environmental stressors?

Answer 6

Salt concentration, temperatures, or pH

Question 7

Where is the enzyme Catalase found?

Answer 7

Found in animals and protists.

Question 8

How does Catalase compare to other enzymes?

Answer 8

Uniquely stable compared to most enzymes, it is more resistant to pH and thermal denaturation.

Question 9

What is Catalase’s optimal environments?

Answer 9

37 degrees C and a 7 pH

Question 10

If you have more enzyme molecules what happens?

Answer 10

More opportunities for substrate-enzyme interactions to occur.

Question 11

Saturation Limit

Answer 11

Once all substrate molecules are bound to available enzyme molecules, increasing the enzyme concentration will not increase the reaction rate.

Question 12

Temperature

Answer 12

A measure of the kinetic energy of the molecules in a system

Question 13

What happens to the enzyme as temperatures decrease?

Answer 13

Enzymes function much slower, the substrate collides with the enzyme less and less.

Question 14

Does low temperatures cause denaturation?

Answer 14

NO, doesn’t cause bonds to break

Question 15

What happens as enzymes increase

Answer 15

the kinetic energy increases therefore increasing the amount of random collisions of enzyme with substrate.

Question 16

What happens if the temperature is higher than the optimal temp?

Answer 16

Enzyme molecules may vibrate and twist rapidly that the hydrogen bonds and other weak attractions will break, disrupting the tertiary structure.

Question 17

How does increased temperatures affect the shape of the enzyme?

Answer 17

Changes the shape of the active site.

Question 18

pH

Answer 18

potential of hydrogen, a measure of the relative number of free hydrogen ions in a substance

Question 19

How does changes in pH affect an enzyme?

Answer 19

Significant chnages in pH alter the shape of an enzyme’s active site by affecting the attractions and repulsions of charges.