enzymes

Question 1

shape of enzymes

Answer 1

3d globular

Question 2

definition of enzymes

Answer 2

biological catalyst, protein in nature, used to speed up or control the rate of a chemical reaction in an organism

Question 3

enzymes are specific to

Answer 3

temperature and PH

Question 4

enzymes are made by what organelle

Answer 4

ribosomes

Question 5

features of enzymes

Answer 5

can be reused
reactions are reversible
not used up in a chemical reaction

Question 6

cell metabolism definition

Answer 6

the sum of total chemical reactions occurring in an organism

Question 7

types of enzymes

Answer 7

anabolic and catabolic

Question 8

substrate definition

Answer 8

substance which the enzyme reacts with

Question 9

product definition

Answer 9

substance the enzyme forms

Question 10

active site definition

Answer 10

part of the enzyme that combines with the substrate

Question 11

enzyme substrate complex definition

Answer 11

the enzyme and substrate joined together

Question 12

optimum activity definition

Answer 12

the conditions at which an enzyme works best at

Question 13

enzyme specificity definition

Answer 13

each enzyme will only react with only 1 particular substrate

Question 14

example of a controlled reaction

Answer 14

SAM

Question 15

optimum temperature humans

Answer 15

37

Question 16

optimum temperature plants

Answer 16

25

Question 17

name a human enzyme and the PH it works best at

Answer 17

pepsin
2
amylase
7

Question 18

denatured enzyme defintion

Answer 18

one that has fully lost its shape and can no longer carry out its function

Question 19

name a plant enzyme and the PH it works best at

Answer 19

catalase
9

Question 20

immobilised enzymes definition

Answer 20

enzymes that are fixed to each other or an inert material

Question 21

bio processing definition

Answer 21

the use of enzyme controlled reactions to produce a product

Question 22

bioreactor definition

Answer 22

a vessel or container in which living cells or their products are used to make a product

Question 23

methods of immobilising enzymes

Answer 23

attached two each other
attached to insoluable supports
enclosed in a membrane or gel

Question 24

advantages of immobilised enzymes

Answer 24

reusable and cost effective
pure product

Question 25

uses of enzymes

Answer 25

penicillin penicillin acylase--> new antibiotic

Question 26

induced fit model theory

Answer 26

substrate combines with active site of enzyme active site slightly changes enzyme substrate complex is formed - the substrate is altered and product is formed product leaves the active site. active site returns to its original shape and can now accept a new substrate molecule

Question 27

enzyme experiment questions : enzyme source of enzyme control experiment

Answer 27

enzyme - catalase source - celery control - unboiled celery experiment - boiled celery (denatured enzyme)

Question 28

how to keep the temperature constant in an enzyme experiment

Answer 28

waterbath with a thermometer

Question 29

purpose of washing up liquid in the enzyme experiments

Answer 29

traps the O2

Question 30

purpose of PH buffer

Answer 30

Keeps the PH constant

Question 31

when drawing the graphs for effect of PH on enzymes and effect of temp, which one should be curved while the other is pointy

Answer 31

temp = curved (at 37) PH = pointy (at 9)

Question 32

Name the substrate and the enzyme that you used

Answer 32

catalase hydrogen peroxide

Question 33

Name the compound you used to harden the gel beads.

Answer 33

calcium chloride

Question 34

Why were the beads rinsed in water before being used?

Answer 34

remove excess yeast

Question 35

Name the compound you used to immobilise the enzyme.

Answer 35

sodium alginate