Genetic engineering Flashcards

(17 cards)

1
Q

How does genetic engineering break the species barrier with example

A

DNA from different species can be joined together
Human genes into bacteria

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2
Q

Tools/Requirements for Genetic engineering

A

DNA Ligase (the glue)
Restriction enzyme (scissors)
Cloning vector
DNA source

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3
Q

Uses/application of genetic engineering for plants

A

Weed killer

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4
Q

Uses/application of genetic engineering for animals

A

Sheep used to produce human clotting factors

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5
Q

Uses/application of genetic engineering for micro-organisms

A

Bacteria used to make insulin

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6
Q

Transgenic organism

A

An organism containing DNA from a different specific

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7
Q

what’s another phrase for a transgenic organism

A

GMO genetically modified organisms

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8
Q

GMO

A

Genetically modified organism

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9
Q

Ethics surrounding genetic engineering

A

Release of GMO into the environment
Use of GMO as a food source
Animal suffering

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10
Q

what is genetic engineering

A

the artificial manipulation or alteration of genes

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11
Q

Steps involved in genetic engineering

A

I can learn to code easily
Isolation
Cutting
Ligation
Transformation
Cloning
Expression

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12
Q

Isolation (genetic engineering)

A

The need to remove the human DNA and target gene from its chromosome and the plasmid DNA from the bacterium

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13
Q

Cutting

A

Human chromosome and bacterial plasmid are cut using the same bacterial enzyme

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14
Q

Ligation

A

Target gene is placed into DNA of plasmid because their ends are complimentary

Dna ligase is used to form strong bonds within the recombinant DNA

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15
Q

Transformation (genetic engineering) n

A

The uptake of DNA into the cell

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16
Q

cloning

A

Produces identical copies of the bacterium containing the recombinant DNA

17
Q

Expression

A

The organism with the recombinant DNA produces the desired product