What is a transgenic organism?
An organism carrying recombinant DNA
What is recombinant DNA?
Genetic material from different sources combined to a single DNA molecule
Why is Taq polymerase used in PCR?
It synthesizes new DNA and it is thermostable, working at high temperatures
5 components mimicked by PCR during replication?
Primers
dNTPs
Buffer
Template DNA
Thermal cycling
What is a genomic library?
The complete genome of an organism divided into recombinant plasmids
How does Sanger sequencing work?
A pool of normal NTPs and tagged NTPs are present. When synthesis occurs, either a normal NTP will be picked up or a ddNTP which terminates the sequence. The fragements are then read from smallest to largest according to the color tags.
How can only exonic sequences be cloned?
Reverse transcriptase of mRNA
Ways to introduce DNA to eukaryotic cells?
Five alternatives
Transfection
Electroporation
Transduction
Microinjection
Conjugation
Enzymatic activity of Cas9?
RNA-guided endonuclease
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Microscopy15
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Microbial Growth18
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Cell Structure21
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Central Dogma I: Transcription4
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Central Dogma III: Translation14
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Central Dogma IV: Regulation14
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Dugga Practice28
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Microscopy II14
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Bacterial Genetics & Transfer of Genetic Material17
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Prokaryotic Diversity30
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Microbial Metabolism17
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Genetic Engineering9
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Genomics19
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Chemotaxis23
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Biofilms / Quorum Sensing18
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Pathogenicity & Host Interactions20
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Antimicrobial Mechanism of Action19
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AMR9
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AMR Factors2
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Alternatives to Antibiotics13
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Eukaryotic Microbes26
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Viruses, phages and prions20
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Bioinformatics12
