Microscopy

Question 1

What does wavelength determine?

Answer 1

The perceived color

Question 2

What does amplitude correspond to?

Answer 2

Brightness or energy

Question 3

What does frequency correspond to?

Answer 3

The rate of wavelength per unit of time

Question 4

A lower frequency has _ wavelength

longer or shorter

Answer 4

longer

Question 5

Translucent objects _ light while opaque objects _ or _ light

Answer 5

Translucent transmits light, opaque reflects or absorbs light

Question 6

A higher refractive index corresponds to _ travel

slower or faster

Answer 6

slower

Question 7

A greater refraction of light is due to …

Answer 7

A larger difference between refractive indexes

Question 8

What is the numerical aperture?

Answer 8

A lens’s ability to capture light (higher = better resolution)

Question 9

What are two factors that affect resolution?

Answer 9

Wavelength (short) and numerical aperture (high)

Question 10

Which color do gram positive bacteria end up after staining and why?

Answer 10

The yremain blue or purple as the CV and iodine is fastened in the thicker cell wall

It rinses away for gram negative bacteria that do not have the thick wall

Question 11

What color are acid-fast bacteria stained?

Answer 11

red

Question 12

Basic stains are used to dye _ charged cells

Answer 12

negatively

Question 13

Acidic stains are used to dye _ charged cells

Answer 13

positively

Question 14

What is an ultramicrotome for?

Answer 14

Cutting very thin slices of cell for use with SEM

Question 15

What is a key difference between fluorescence and phosphorescence?

Answer 15

fluorescence is immediate while phosphorescence is delayed due to a transition state