Genomics

Question 1

What is genomics?

Answer 1

The study of the genome in its entirety

Question 2

What is transcriptomics?

Answer 2

The study of the transcriptome (RNA produced in the cell)

Question 3

What is proteomics?

Answer 3

The study of proteins in a cell

Question 4

What is metabolomics?

Answer 4

The study of chemical processes of metabolites

Question 5

What is an example of first generation genomics?

Answer 5

Sanger sequencing

Question 6

What is the method of Sanger sequencing?

Answer 6

ddNTPs are introduced which compete for binding with dNTPs. Binding of a ddNTP leads to chain termination, and a fluorescent reaction that is analyzed in a detector

Question 7

Why do viruses and bacteria have less genes?

Answer 7

Lack of non-coding genes

Question 8

What are two methods of studying RNA?

Answer 8

RNA-seq
ChIP sequencing

Question 9

What are two methods of studying proteins in an organism?

Answer 9

SDS-page
Mass spectrometry
ELISA, western blotting
3D structure analysis

Question 10

The main four “-omics”?

Answer 10

Genomics
Transcriptomics
Proteomics
Metabolomics

Question 11

What does metabolomics cover?

Four alternatives

Answer 11

sugars
nucleotides
amino acids
lipids

Question 12

Bacteria have a diploid genome

True/False

Answer 12

False
Haploid, only one copy

Question 13

What is a pangenome?

Answer 13

The genes present in all strains of a species, including core and accessory

Question 14

2nd gen sequencing method?

Answer 14

Massively parallel sequencing

Question 15

3rd gen sequencing method?

Answer 15

single molecule real time (SMRT)

Question 16

What is de novo sequencing?

Answer 16

Sequencing a new genome that has not been previously mapped

Question 17

What does ChIP-seq do?

Answer 17

Identify and map where proteins bind to DNA in the genome

Question 18

Overview of ELISA?

Answer 18

Enzyme-linked immunosorbent assay

An antigen is bound to a surface. A complementary antibody attached to an enzyme is added and binds to the antigens. When substrate is added, it interacts with the enzyme causing a color change which is read with a spectrophotometer

Question 19

Overview of Western blot?

Answer 19

SDS-PAGE to separate proteins. Transfer to membrane. Add antibody that recognizes target protein, followed by conjugate antibody linked with enzyme. Color developed at target protein