Light microscope maximum magnification
1000X
Uses an annular ring to enable contrast
phase-contrast microscopy
Types of light microscopy?
7 alternatives
brightfield
darkfield
phase-contrast
differential interference contrast
fluorescence
confocal
two-photon
Image on bright background
brightfield microscopy
Bright objects on dark background
darkfield microscopy
Laser scans on multiple z-planes
confocal microscopy
Maxiumum magnification of electron microscopy?
100,000X
Two types of electron microscopy?
transmission electron microscopy
scanning electron microscopy
3D appearance
electron microscopy
SEM
Probe passed above sample creates electic current
Scanning tunneling microscopy
Maxiumum magnification of STM?
100,000,000X!!!
Probe measures force of atoms
atomic force microscopy
Steps in Gram staining?
4 steps
Crystal violet
Iodine
Alcohol
Safranin
What do acid-fast stains differentiate?
bacteria with mycolic acids