Lab Final - Appendix

Question 1

Which side of the petri dish should you label?

Answer 1

agar side (smaller)

Question 2

What should go on the label of the petri dish?

Answer 2

name, lab section, TA name, description of the culture

Question 3

Why is the Petri dish stored bottom side up?

Answer 3

To prevent condensation from falling onto the culture

Question 4

Do you need a new inoculating loop for each third of streaking?

Answer 4

Yes

Question 5

How do you streak with a wooden applicator?

Answer 5

Divide petri dish into 4 quadrants, 2 of them wt and unk. only do ONE LINE in each quadrant

Question 6

Which magnification to start with using a microscope?

Answer 6

4X, lowest, then 10X, then 40X

Question 7

Why don’t we use the 100X objective lens?

Answer 7

It is an oil lens, needs immersion oil to improve its resolution and clarity sufficiently

Question 8

Different types of micropipettes have a different range (uL) they can handle. What is the range and tip for

P2
P10
P20
P100
P1000

Answer 8

P2, 0.2-2.0, small, clear
P10, 1-10, small, clear
P20, 2-20, medium, yellow
P100, 10-100, medium, yellow
P1000, 100-1000, large, blue

Question 9

The top line of a micropipette shows what

Answer 9

The maximum range

P20 2-20
The top one, if it’s 2, represents 20 uL

Question 10

When you’re drawing up liquid using a micropipette, which stop do you go to?

Answer 10

First stop

Question 11

When you’re expelling liquid using a micropipette, which stop do you go to?

Answer 11

Second stop

Question 12

What is the phenotypic difference between female and male Drosophila?

Answer 12

females are striped, pointed abdomen, large

males have one large black chunk at the end, round abdomen, small

Question 13

What are vestigial wings?

Answer 13

abnormal/almost all gone