Lab Final - Lab 9 (Polymorphism)

Question 1

What are the 3 different types of polymorphisms?

Answer 1

Base substitutions, such as

restriction fragment length polymorphism (RFLPs) or single nucleotide polymorphisms (SNPs)

or

transposable element (a piece of DNA that can move to different places in a genome, such as Alu)

Question 2

How can the presence or absence of Alu at PV92 be detected?

Answer 2

PCR, which uses forward and reverse primers that bind sequences flanking the locus

amplifies the DNA segments producing DNA fragments of different lengths for each allele. 416 bp if Alu is absent, 731 bp if Alu is present

Question 3

Individuals homozygous for Alu will have…

Answer 3

731 bp product

Question 4

Those homozygous without Alu will have…

Answer 4

416 bp product

Question 5

Heterozygous individuals for Alu will…

Answer 5

produce one 731 and one 416 bp product