Lecture 28

Question 1

RBC membranes contain what?

Answer 1

glycoproteins and glycolipids that act as antigens

Question 2

Patients cannot receive blood with antigens they don’t already have, or they will produce what?

Answer 2

antibodies, cause agglutination, cause danger

Question 3

What is removed from donated blood before transfusion?

Answer 3

WBCs

Question 4

Where is the ABO gene located? Expressed?

Answer 4

9q34

immature RBCs

Question 5

What does the ABO gene encode?

Answer 5

enzyme (glycosyltransferase) that attaches monosaccharides to the H antigen (target oligosaccharide)

Question 6

What does IA, IB, and i do?

Answer 6

functional enzyme adds A sugar

functional enzyme adds B sugar

frameshift deletion, no functional enzyme, only H antigen

Question 7

How does IA and IB differ?

Answer 7

at 7 base pairs (4 missense mutations, 3 silent mutations)

Question 8

i is same as IA except what

Answer 8

single bp deletion (frameshift)

Question 9

IA and IB are what

Answer 9

codominant

Question 10

What are the antigens present in IA/IA or IA/i?

What is the blood type?

Answer 10

A and H

A

Question 11

What are the antigens present in IB/IB or IB/i?

What is the blood type?

Answer 11

B and H

B

Question 12

What are the antigens present in IA/IB?

What is the blood type?

Answer 12

A, B, H

AB

Question 13

What are the antigens present in i/i?

What is the blood type?

Answer 13

H only

O

Question 14

Patients can receive what?

Answer 14

antigens they already have

Question 15

What can donate to A

Answer 15

A O

Question 16

What can donate to B

Answer 16

B O

Question 17

What can donate to AB

Answer 17

A, B, AB, O

Question 18

What can donate to O?

Answer 18

O (universal donor)

Question 19

What does rhesus do? Where is it located?

Answer 19

RHD (1p36), encodes D antigen (membrane protein)

Question 20

D/_ is what

Answer 20

Rh+

Question 21

d/d is what

Answer 21

Rh-

Question 22

d is a what allele

Answer 22

amorphic

Question 23

What is the pregnancy concern of rhesus?

Answer 23

Rh- mom and Rh+ fetus cause hemolytic disease

moms immune system might attack fetal Rh+ RBCs

Question 24

What encodes the MN blood group? Where is this located? What are the AA associated with M and N

Answer 24

GYPA encodes a membrane protein

4q31

M ser and gly

N leu and glu

Question 25

M and N are what

Answer 25

codominant

Question 26

How is blood currently tested?

Answer 26

antibodies are mixed with blood samples if agglutination occurs, antigen is present