what are antiviral frugs developed for so far
viruses that cause significant morbidity or mortality
do antiviral drugs give severe side effects
yes
what do antiviral drugs target
unique viral prossess (RdRp/proteases)
why dont you want to target host for antiviral drugs
more symtoms
3 targets for antiviral drugs
attachement
penetration and uncoating
mRNA synthesis
what is a cell or mechanisms based screen for
to assess wether your inhibitor is inhibiting what you want it to
first part of collaborative antiviral drugs
virlogy
biochemistry
second part of collaborative antiviral drugs
chemistry and pharmacology
enzyme activity based screen (how does it work)
drug to inhibit protease cleavage - less fluorescence means drug is working to inhibit protease
virus replication based screens
real live viruses and adding drugs to see I they stop viral replication
high throughput methods (3)
virus replication screens
enzyme activity based screens
synthetic screens
4 phases of drug trials
phase 1- safety
phase 2 - efficacy
phase 3 - efficacy and side effects
phase 4 - after FDA approval (still looking for side effects)
goal of frug resistance
completely block viral replication so they dont become resistant to the drug
targeting genome replication inhibits what
viral polymerases
prime targets for ant-viral drugs (3)
DNA polymerase
reverse transcriptase
RdRp
3 drugs that inhibit structures needed for replication
acyclovir
AZT
Ribavirin
resistence to antiviral drugs is an issue due to what
mutation of the virus
combination therapy
using two drugs instead of one that target different parts of the virus life cycle
