where does transcription happen for DNA
nucleus
what RNA polymerase makes mRNA
2
what RNA polymerase makes tRNA
3
what DNA viruses are the exception and transcribe in the cytoplasm
mimivirus
poxvirus
what is one thing that needs to happen to DNA before it can be transcribed into mRNA
it needs to be double stranded
methylation of histones is
repressive
acetylation of histones is
activating
what happens to dsDNA in the nucleus
gets packaged into chromatin
start site for RNA polymerase
TATA sequence + initiator sequence
order for expressing mRNA temporal cascade from DNA viruses
immediate early gene - regulators
early gene - replication machinery
late gene - structural proteins for packaging
2 options for the protein related from dsDNA?
can either turn on transcription of another gene or the same gene
whats required for late gene expression
DNA replication
regulation models for late gene transcription after DNA replication (4)
titration of cellular transcriptional repressors
viral activators of late transcription
transcription from simple promotors (RNA polymerase 2)
regulation of transcription termination (changes structure)
what drives expression of most late teens after DNA replication
MLP (major late promoter)
describe genome packaging
replicated viral DNA is recognized by packaging signals ad loaded into capsids by viral packaging machinery
