Micro

Question 1

acetamide - carbon

Answer 1

+ pseudomonas
- e coli

Question 2

acetate - carbon

Answer 2

+ e coli
- shigella

Question 3

L-Ala-AMC

Answer 3

+ e coli
- staph aureus

Question 4

Bacitracin

Answer 4

+ Strep pyogenes
- Strep agalactiae

Question 5

Bile esculin

Answer 5

+ Strep bovis, group D strep, enterococcus

Question 6

bile solubility (deoxycholate)

Answer 6

+ strep pneumonia
- strep viridans, enterococcus faecalis

Question 7

butyrate (catarrhalis)

Answer 7

+ moraxella catarrhalis - blue
- n gonorrheae

Question 8

CAMP test

Answer 8

+ strep agalactiae - arrow formation
- strep pyogenes

Question 9

catalase

Answer 9

+ staph
- strep

Question 10

cetrimide

Answer 10

+ pseudomonas aeruginosa

Question 11

citrate

Answer 11

+ klebsiella aerogenes
- e coli

Question 12

coagulase

Answer 12

+ staph aureus

Question 13

Deoxyribonucleic acid hydrolysis - deoxyribonuclease

Answer 13

+ serratia - enterobacter
+ staph aureus - other staph
+ m. catarrhalis - neiserria

Question 14

esculin hydrolysis

Answer 14

+ enterococcus faecalis
- e coli, strep pyogenes

Question 15

gelatin hydrolysis

Answer 15

+ b. subtilis, staph, enterobacterales
- e coli

Question 16

growth at 42

Answer 16

+ pseudomonas aeruginosa
- pseudomonas fluorescens

Question 17

hippurate

Answer 17

+ strep agalactiae
- strep pyogenes

Question 18

indole - tryptophanase

Answer 18

kovac’s
+ e coli
- kleb pneumonia

ehrlich’s
+ h. influenzae
- h. parainfluenza

ehrlich’s (anaerobic)
+ porphyromonas
- b. fragilis

Question 19

leucine aminopeptidase

Answer 19

+ enterococcus faecalis
- aerococcus viridans

Question 20

microdase

Answer 20

+ micrococcus luteus
- staph aureus

Question 21

lactobacillus MRS broth

Answer 21

+ lactobacillus lactis
+ with gas leuconostoc sp.
- e coli

Question 22

4- methylumbelliferyl-B-d-glucuronide test

Answer 22

+ e coli
- klebsiella pneumoniae

Question 23

nitrite reduction

Answer 23

+ proteus mirabilis - no color change
- acinetobacter bamanii - red

Question 24

o-nitrophenol-B-D-galactopyranoside

Answer 24

+ shigella sonnei - yellow
- salmonella enterica

Question 25

optochin

Answer 25

+ strep pneumoniae 14mm - strep pyogenes

Question 26

oxidase (Kovac’s)

Answer 26

+ pseudomonas aeruginosa - purple - e coli

Question 27

oxi and fermentation

Answer 27

fermenter - e coli oxidizer - pseudomonas aeruginosa

Question 28

phenylalanine deaminase

Answer 28

+ morganella, proteus, providencia - green slant

Question 29

l-pyrrolidonyl arylamidase (PYR yellow to red)

Answer 29

+ enterococcus faecalis, strep pyogenes - strep agalactiae

Question 30

pyruvate (green to yellow)

Answer 30

+ e faecalis - e faecium

Question 31

salt tolerance (purple to yellow)

Answer 31

+ enterococcus

Question 32

spot indole - tryptophanase, blue color in 20sec

Answer 32

+ e coli - klebsiella

Question 33

X and V factor

Answer 33

XV - H influenzae XV, V - H parainfluenzae XV, X - H ducreyi

Question 34

urease (christensen’s) - magenta

Answer 34

+ proteus vulgaris weak + klebsiella pneumoniae - e coli

Question 35

tinsdale agar

Answer 35

c diphtheriae

Question 36

elek plate

Answer 36

c diphtheriae

Question 37

umbrella motility

Answer 37

Listeria

Question 38

reverse CAMP

Answer 38

C perfringens a-toxin (S. agalactiae camp factor) Arcanobacterium haemolyticum

Question 39

bulls eye colony

Answer 39

Yersinia enterocolitica

Question 40

green metallic sheen on EMB

Answer 40

e coli

Question 41

acinetobacter color on macconkey

Answer 41

purple

Question 42

burkholderia cepacia color on chocolate agar

Answer 42

green

Question 43

satellite phenomenon

Answer 43

H. influenzae around S. aureus

Question 44

cut glass internal granular speckling

Answer 44

legionella pneumophila

Question 45

double zone of beta hemolysis

Answer 45

C. perfringens

Question 46

bread crumb-like colonies

Answer 46

Fusobacterium nucleatum and actinomyces israelli (molar tooth)