Chromatography Lab

Question 1

Chlorophyll A color

Answer 1

greenish-blue

Question 2

Chlorophyll B color

Answer 2

green

Question 3

Carotenoids color

Answer 3

orange-yellow

Question 4

Xanthophylls color

Answer 4

yellow

Question 5

What is chromatography

Answer 5

a process used to separate a mixture of substances into individual parts

Question 6

What 2 principles is chromatography based on?

Answer 6

-the ability of different substances to dissolve to different degrees in the same solvent

-the ability of some substances being able to hydrogen-bond to the chromatography paper better than others

Question 7

What happens to the least soluble substances

Answer 7

it will appear sooner

Question 8

What affect does hydrogen-bonds have on chromatography

Answer 8

if a substance bonds better it will show earlier

Question 9

What is the Rf formula

Answer 9

distance of pigment
——————
Distance of solvent

Question 10

What 2 qualities make things go last

Answer 10

high solubility and low H-bonds to paper

Question 11

What 3 conditions make fall leaves not green

Answer 11

1. Less light
2. Less heat
3. Less moisture