1
Q
What is urea hydrolysis testing?
A
Urea hydrolysis testing is a test used to differentiate bacteria based on if they possess the enzyme urease.
2
Q
What is the basis of urea hydrolysis testing?
A
The basis of urea hydrolysis testing is that they hydrolysis of urea produces ammonia and CO2 and the ammonia alkalizes the medium which is detected by the pH indicator phenol red, causing a colour change from light orange to pink.
Laboratory media and tests.
flashcards
Decks in class (34)
# Cards
-
Differential and selective media.2
-
Chromogenic agar.2
-
MacConkey agar.2
-
Blood agar.2
-
Chocolate agar.2
-
EMB agar.2
-
SS agar.2
-
Mannitol salt agar.2
-
Hektoen agar.2
-
M-tergitol 7 agar.2
-
XLD agar.2
-
CLED agar.2
-
SDA agar.2
-
Citrate agar.2
-
Urea agar.2
-
Nutrient agar.2
-
Media solidity.4
-
Gram staining.2
-
Morphology observation2
-
Motility testing.2
-
Indole utilisation testing.2
-
Methyl red testing.2
-
Voges-Proskauer testing.2
-
Citrate utilisation testing.2
-
H2S production testing.2
-
Glucose fermentation testing.2
-
Catalase testing.2
-
Oxidase testing.2
-
Gelatine hydrolysis testing.2
-
Urea hydrolysis testing.2
-
Nitrate reduction testing.2
-
Sterility testing.6
-
Bioburden assessments.7
-
Endotoxin testing.2
